Abstract
Glutathione-S-transferase (EC 2.5.1.18) activity was assayed in hepatic and extra-hepatic tissues of pigeons using l-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene as substrates. Gluthathione-S-transferase activity towards 1-chloro-2,4-dinitrobenzene in pigeon was in the order: kidney > liver > testes > brain > lung> heart. The enzyme activity with 1-chloro-2,4-dinitrobenzene as substrate was 40–44 times higher in pigeon liver and kidney than that observed with 1,2-dichloro-4-dinitrobenzene as substrate.K m values of hepatic and renal glutathione transferase with l-chloro-2,4-dinitrobenzene as substrate were 2.5 and 3 mM respectively. Double reciprocal plots with varying reduced gluthathione concentrations resulted in biphasic curves with twoK m values (liver 0.31 mM and 4mM; kidney 0.36 mM and 1.3 mM). The enzyme activity was inhibited by oxidized gluthathione in a dose-dependent pattern. 3-Methylcholanthrene elicited about 50% induction of hepatic glutathione transferase activity whereas phénobarbital was ineffective.
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Abbreviations
- Chloro-dinitrobenzene:
-
l-chloro-2, 4-dinitrobenzene
- dichlorodinitrobenzene:
-
1,2-dichloro-4-nitrobehzene
- GSH:
-
gluthathione (reduced)
- GSSG:
-
glutathione (oxidized)
- 3MC:
-
3-methyl-cholanthrene.
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Kumar, A., Husain, M.M., Mukhtar, H. et al. Hepatic and extra-hepatic glutathione-S-transferase activity in wild pigeons (Columba livia). J. Biosci. 2, 181–189 (1980). https://doi.org/10.1007/BF02703244
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DOI: https://doi.org/10.1007/BF02703244