Abstract
Glutathione-S-transferase (EC 2.5.1.18) activity was assayed in hepatic and extra-hepatic tissues of pigeons using l-chloro-2,4-dinitrobenzene and 1,2-dichloro-4-nitrobenzene as substrates. Gluthathione-S-transferase activity towards 1-chloro-2,4-dinitrobenzene in pigeon was in the order: kidney > liver > testes > brain > lung> heart. The enzyme activity with 1-chloro-2,4-dinitrobenzene as substrate was 40–44 times higher in pigeon liver and kidney than that observed with 1,2-dichloro-4-dinitrobenzene as substrate.K m values of hepatic and renal glutathione transferase with l-chloro-2,4-dinitrobenzene as substrate were 2.5 and 3 mM respectively. Double reciprocal plots with varying reduced gluthathione concentrations resulted in biphasic curves with twoK m values (liver 0.31 mM and 4mM; kidney 0.36 mM and 1.3 mM). The enzyme activity was inhibited by oxidized gluthathione in a dose-dependent pattern. 3-Methylcholanthrene elicited about 50% induction of hepatic glutathione transferase activity whereas phénobarbital was ineffective.
Similar content being viewed by others
Abbreviations
- Chloro-dinitrobenzene:
-
l-chloro-2, 4-dinitrobenzene
- dichlorodinitrobenzene:
-
1,2-dichloro-4-nitrobehzene
- GSH:
-
gluthathione (reduced)
- GSSG:
-
glutathione (oxidized)
- 3MC:
-
3-methyl-cholanthrene.
References
Bend, J. R., Smith, B. R., Ball, L. M. and Mukhtar, H. (1979) inConjugation reactions in drug biotransformation, ed. A. Aitio (Amsterdam, North Holland. New York: Elsivier Press) p. 3.
Cantfort, J. V., Manil, L., Gielen, J. E., Glatt, H. R. and Oesch, F. (1979).Biochem. Pharmacol,28, 455.
Chasseaud, L. F. (1976) inGlutathione: Metabolism and function, eds. I. M. Arias and W. B. Jakoby (New York: Ravan Press) p. 77.
Guddewar, M. B. and Dauterman, W. C. (1979)Phytochemistry,18, 735.
Habig, W. H., Palist, M. J. and Jakoby, W. B. (1974)J.Biol Chem.,249, 7130.
Hayakawa, T., Udenfriend, S., Yagi, H. and Jerina, D. M. (1975)Arch. Biochem. Biophys.,170, 438.
Husain, M. M., Kumar, A. and Mukhtar, H. (1979)Indian J. Biochem. Biophys. (in press).
Jakoby, W. B. (1978)Adv. Enzymol,46, 383.
James, M. O., Bowen, E. R., Dansette, P. M. and Bend, J. R. (1979)Chem. Biol Interact.,25, 321.
Jerina, D.M. and Bend, J. R. (1977) inBiological reactive intermediates. Formation, toxicity and inactivation, eds D. Jollow, J. J. Kocsis, R. Snyder and H. Vainjo (New York: Plenum Press) p. 207.
Kaplowitz, N., Clifton, G., Kuhlenkamp, J. and Wallin, J. D. (1976)Biochem. J. 158, 243.
Lowry, D. H., Rosenbrough, N. J., Farr, A. L. and Randall, R. J. (1951)J. Biol. Chem.,193, 265.
Motoyama, N., Kulkarni, A. P., Hodgson, E. and Dauterman, W. C. (1978),Pestic. Biochem. Physiol,9. 255.
Mukhtar, H. and Bend, J. R. (1977)Life Sci.,21, 1277.
Mukhtar, H. and Bresnick, E. (1976a)Biochem. Pharmacol.,25, 1081.
Mukhtar, H. and Bresnick, E. (1976b)Chem. Biol. Interact.,15, 59.
Mukhtar, H., Philpot, R. M. and Bend, J. R. (1978)Biochem. Biophys. Res. Commun.,81, 89.
Nimmo, I. A., Clapp, J. B. and Strange, R. C. (1979)Comp. Biochem. Physiol,63, 423.
Pegg, D. G. and Hook, J. B. (1976)J. Pharmacol. Exp. Ther.,200, 65.
Runnels, J. M. and Khan, M. A. Q. (1975)Gen. Pharmacol,6, 97.
Sifri, M., Sell, J. L. and Davison, K. L. (1975)Comp. Biochem. Physiol,51, 213.
Stenersen, J., Gutherberg, C. and Mannervik, B. (1979)Biochem J.,181, 47.
Wit, J. G. and Snel, J. (1968)Eur. J. Pharmacol,3, 370.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kumar, A., Husain, M.M., Mukhtar, H. et al. Hepatic and extra-hepatic glutathione-S-transferase activity in wild pigeons (Columba livia). J. Biosci. 2, 181–189 (1980). https://doi.org/10.1007/BF02703244
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02703244